- In "The Order of the Stone", Jesse's Gang receives eight Slimeballs from Ivy, the Slime Vendor in EnderCon. Lukas later gives his Slimeball to Jesse, allowing them to craft a Slimeblock on a Crafting Table.
- In "A Block and a Hard Place", Jesse uses a Slimeball and a Piston found in a chest at the end of Ivor's Maze to craft a Sticky Piston.
- In "A Portal to Mystery", CaptainSparklez is shown to have a slime ball in his inventory.
- In "A Journey's End?", Lukas will drop a slimeball when he dies in the Spleef arena.
- Ivy mentions that the slime ball supply is limited and that she can only give 2 per customer. If she broke the rules, she would have to do it for everyone else.
- In Minecraft, Slimeballs are dropped by Slimes.
- It requires nine Slimeballs to craft a Slime Block in Minecraft and Minecraft: Story Mode.
- CaptainSparklez is known to possess a Slimeball in his inventory.
- This is a possible reference to Jerry The Slime.